DNA Preparation and Amplification on Plastic Microfluidic Devices for Genetic Analyses.Abstract: "The utilization of a microfluidics platform for DNA analysis would provide numerous advantages over conventional DNA analysis methods, including a completely closed system and reduction in analytical time. Before DNA can be analyzed, cells must first be eluted from the substrate (e.g., cotton swabs). A novel buffer is described, increasing sperm cell recovery from a cotton swab matrix to 85-90 %, an ~2-fold increase in recovery compared to a traditional differential extraction buffer, while comprehensively lysing epithelial cells, thereby increasing the probability of generating a full male DNA profile. In addition, to eliminate multiple sample handling steps and binding DNA to silica for purification (which leads to irreversible binding, thus, loss of DNA), an enzyme-based DNA preparation method is characterized, optimized and applied to various samples, including buccal swabs and blood stains. This method provides PCR-ready DNA in approximately 20 minutes, an ~4-fold reduction in preparation time compared to conventional DNA extraction."
Jenny Ann Lounsbury
University of Virginia
Department of Chemistry
|Date Added||November 5, 2016|
|Date Modifed||October 17, 2017|
|Collection||UVA scholarship on sexual violence, 1974-|
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