DNA Preparation and Amplification on Plastic Microfluidic Devices for Genetic Analyses.
Abstract: "The utilization of a microfluidics platform for DNA analysis would provide numerous advantages over conventional DNA analysis methods, including a completely closed system and reduction in analytical time. Before DNA can be analyzed, cells must first be eluted from the substrate (e.g., cotton swabs). A novel buffer is described, increasing sperm cell recovery from a cotton swab matrix to 85-90 %, an ~2-fold increase in recovery compared to a traditional differential extraction buffer, while comprehensively lysing epithelial cells, thereby increasing the probability of generating a full male DNA profile. In addition, to eliminate multiple sample handling steps and binding DNA to silica for purification (which leads to irreversible binding, thus, loss of DNA), an enzyme-based DNA preparation method is characterized, optimized and applied to various samples, including buccal swabs and blood stains. This method provides PCR-ready DNA in approximately 20 minutes, an ~4-fold reduction in preparation time compared to conventional DNA extraction."| Creator |
Jenny Ann Lounsbury
|
|---|---|
| Publisher |
University of Virginia
|
| Date |
2012
|
| Type |
Dissertation
|
| Advisor |
James Landers
Ralph Allen
Linda Columbus
David Cafiso
|
|---|---|
| Department |
Department of Chemistry
|
| Date Added | November 5, 2016 |
|---|---|
| Date Modifed | October 17, 2017 |
| Collection | UVA scholarship on sexual violence, 1974- |
Item Relations
This item has no relations.