Analytical advances in forensic science: Development of microfluidic systems for analysis of biological evidence.
Abstract: "With sexual assault evidence, conventional differential extraction is utilized to obtain separate male and female fractions of DNA, from which a profile from the victim and perpetrator can be delineated. Chapter 2 discusses the development of a microscale method to replace the conventional differential extraction based upon the different physicochemical properties of the two cell types. Chapter 3 focuses on the development of a quantitative PCR (gPCR) method for the simultaneous quantitation of male and total autosomal DNA in a biological sample as a means to evaluate the results of the cell sorting methods described here. Chapter 4 describes the development of a second means of obtaining separate male and female fractions of DNA from sexual assault evidence, this time exploiting ultrasonic energy to selectively trap cells in a flow stream in a microdevice. This 'acoustic trapping', in combination with simplistic, but effective, valving via precise flow control resulted in separation of the sperm cell and female DNA fractions."| Creator |
Katie Maree Horsman
|
|---|---|
| Publisher |
University of Virginia
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| Date |
2007
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| Type |
Dissertation
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| Advisor |
James P. Landers
|
|---|---|
| Department |
Department of Chemistry
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| Date Added | November 5, 2016 |
|---|---|
| Date Modifed | October 17, 2017 |
| Collection | UVA scholarship on sexual violence, 1974- |
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